Composite

Part:BBa_K5047040:Design

Designed by: Clément Alain Serge Corneil Vanmerris   Group: iGEM24_UNILausanne   (2024-10-01)


This sequence enables constitutive Dicer expression in yeast.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1845
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 796
    Illegal BglII site found at 992
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1950
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 160


Design Notes

The DICR coding sequence was divided into two segments, ordered as g-blocks from IDT. It was designed with overlapping regions between g-blocks, the promoter, and the terminator facilitating the efficient assembly of the full genetic sequence.


Source

The pTEF1 promoter originates from the pSP329 plasmid provided by Prof. Serge Pelet (DMF, University of Lausanne). Both the DICR coding sequence and the tSTE2 terminator were synthetically designed to ensure optimal compatibility and performance in yeast.

References